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Schaeffer–Fulton stain
The Schaeffer–Fulton stain is a technique designed to isolate endospores by staining any present endospores green, and any other bacterial bodies red.〔(Definition:Schaeffer-Fulton Stain )〕 The green stain is malachite green, and the counterstain is safranin, which dyes any other bacterial bodies red.
==Procedure==
Using an aseptic technique, bacteria are placed on a slide and heat fixed. The slide is then suspended over a water bath with some sort of porus paper over it, so that the slide is steamed. Malachite green is applied to the slide, which can penetrate the tough walls of the endospores, staining them green. After five minutes, the slide is removed from the steam, and the paper towel is removed. After cooling, the slide is rinsed with water for thirty seconds. The slide is then stained with diluted safranin for two minutes, which stains most other microorganic bodies red or pink. The slide is then rinsed again, and blotted dry with bibulous paper.〔Harley and Prescott: ''Laboratory Exercises in Microbiology'', page 58. McGraw Hill, 2002.〕
After drying, the slide can then be viewed under a light microscope.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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